cFos immunoreactivity is enhanced with biotin amplification.
نویسندگان
چکیده
منابع مشابه
Iba1 immunoreactivity is enhanced following an antigen retrieval treatment with EDTA, pH 6.0
Antigen retrieval is a standard procedure to enhance immunohistochemical detection. However, among the many choices of techniques available for antigen retrieval, it is important to choose a method that works specifically for the antibody of interest. The small calcium binding protein, Iba1, has been well characterized as a microglia specific marker useful for identifying both resting and activ...
متن کاملBiotin Auxotrophy and Biotin Enhanced Germ Tube Formation in Candida albicans
Due to the increased number of immunocompromised patients, infections with the pathogen Candida albicans have significantly increased in recent years. C. albicans transition from yeast to germ tubes is one of the essential factors for virulence. In this study we noted that Lee's medium, commonly used to induce filamentation, contained 500-fold more biotin than needed for growth and 40-fold more...
متن کاملDefinition of three serotypes of Hantaviruses by a double sandwich ELISA with biotin-avidin amplification system.
A total of seven different isolates of Hantaviruses from five mammalian species and six geographical areas were examined by a double sandwich ELISA with biotin-avidin amplification system. Three serotypes were recognized by comparing antigen titres to homologous and heterologous antibody and blocking antibody titres to homologous and heterologous antigens. Serotype 1 included four strains: Hant...
متن کاملSelective amplification via biotin- and restriction-mediated enrichment (SABRE), a novel selective amplification procedure for detection of differentially expressed mRNAs (circadian expressionycompetitive hybridizationycoumarin 7-hydroxylaseymagnetic separationystreptavidin-biotin affinity)
We present a novel subtractive enrichment protocol for the identification of differentially expressed mRNA species. This procedure, called SABRE (selective amplification via biotinand restriction-mediated enrichment), uses selective streptavidin-biotin affinity and restriction enzyme site reconstitution to enrich for cDNA species more abundant in one population than in another. Analysis of live...
متن کاملGenetic Transformation of Oat Mediated by Agrobacterium is enhanced with Sonication and Vacuum Infiltration
Background: Oat (Avena sativa) with high nutritive value and fiber content is used as the main food grain in many countries for human diet as well as animal feed. Recently, it became difficult to transfer new genes through the conventional breeding due to the lack of desirable traits. Objectives: The current study aimed at achieving a st...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1994
ISSN: 0022-1554,1551-5044
DOI: 10.1177/42.12.7983364